Question Number: 348
UMUC Biology 102/103Lab 2: The Chemistry of LifeINSTRUCTIONS:•On your own and without assistance, complete this Lab 2Answer Sheet electronically and submit it via the Assignments Folder by the date listed intheCourse Schedule (under Syllabus).•To conduct your laboratory exercises, use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering the questions. •Save your Lab 2Answer Sheet in the following format: LastName_Lab2 (e.g., Smith_Lab2).•You should submit your document as a Word (.doc or .docx) or Rich Text Format (.rtf) file for best compatibility. Pre-Lab Questions1.Nitrogen fixation is a natural process by which inert or unreactive forms of nitrogen are transformed into usable nitrogen. Why is this process important to life?2.Given what you have learned about the hydrogen bonding shared between nucleic acids in DNA, which pair is more stable under increasing heat: adenine and thymine, or cytosine and guanine? Explain why.3.Which of the following is not an organic molecule; Methane (CH4), Fructose(C6H12O6), Rosane (C20H36), or Ammonia (NH3)? How do you know? Experiment 1: Testing for ProteinsData Tables and Post-Lab AssessmentTable 1: A Priori PredictionsSampleInitial ColorFinal ColorIs Protein Present?1. Albumin Solution2. Gelatin Solution3. Glucose4. Water5. UnknownSampleInitial ColorFinal ColorIs Protein Present?1. Albumin Solution2. Gelatin Solution3. Glucose4. Water5. UnknownTable 2: Testing for Proteins ResultsPost-Lab Questions1.Write a statement to explain the molecular composition of the unknown solution based on the results obtained during testing with each reagent.2.How did your a priori predictions from Table 1 compare to your actual results in Table 2? If there were any inconsistencies, explain why this occurred. 3.Identify the positive and negative controls used in this experiment. Explain how each of these controls are used, and why they are necessary to validate the experimental results.4.Identify two regions which proteins are vital components in the human body. Why are they important to these regions?5.Diet and nutrition are closely linked to the study of biomolecules. Describe one method by which you could monitor your food intake to ensure the cells in your body have the materials necessary to function. Experiment 2: Testing for Reducing SugarsData Tables and Post-Lab AssessmentTable 3: Testing for Reducing Sugars ResultsSampleInitial ColorFinal ColorReducing Sugar Present1 - Potato2 - Onion3 - Glucose Solution4 - Water5 - UnknownPost-Lab Questions1.What can you conclude about the molecular make-up of potatoes and onions based on the test you performed? Why might these foods contain these substance(s)?2.What results would you expect if you tested ribose, a monosaccharide, with Benedict’s solution? Biuret solution?Experiment 3: What Household Substances are Acidic or Basic?Data Tables and Post-Lab AssessmentTable 4: pH Values of Common Household SubstancesSubstancepH PredictionpH Test Strip ColorPost-Lab Questions1.What is the purpose of determining the pH of the acetic acid and the sodium bicarbonate solution before testing the other household substances?2.Compare and contrast acids and bases in terms of their H+ ion and OH- ion concentrations.3.Name two acids and two bases you often use.
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